Singapore
Light
Microscopy
Facilities
Network
OUR NETWORK
Each microscopy core facility has a range of equipment designed to meet the needs of the research community that makes up their user base. A wide variety of different modes of opertion exist, from free access for a limited group of users to open facilities with tiered charging systems. Find out more about each facility below.
Microscopy Unit
Institute of Medical Biology
A*STAR
IMU is a core technology platform of the Institute of Medical Biology, A*STAR whose mission is to provide light and electron microscopy equipment along with image processing and analysis software and the expertise to facilitate research in molecular, cell and developmental biology.
As well as standard widefield and confocal microscopy IMU focuses on seven areas of advanced imaging:
(1) 3D imaging
(2) F-techniques
(3) High content imaging
(4) Live-cell imaging
(5) Super-resolution microscopy
(6) Electron microscopy
(7) Image analysis & processing
We are freely accessible to the IMB researchers and are open to outside useage on a tiered chargable basis.
Contact:
Dr Graham Wright (Head, Microscopy Unit)
Email: graham.wright@imb.a-star.edu.sg
Phone: +65 6407 0167
Find out more on our website here.
Advanced Imaging Laboratory
Life Sciences Institute, NUS
The Advanced Imaging Laboratory is a core microscopy facility of the Life Sciences Institute. Light microscopes in the facility are available for fluorescence, live-cell timelapse and confocal imaging. These are complemented with specialised software for processing and analysis of multi-dimensional images
Microscopes:
(1) Leica SP5 inverted confocal microscope
(2) Zeiss LSM510 upright confocal microscope
(3) Zeiss AxioImager Z1 upright fluorescence microscope
(4) Olympus IX81 inverted fluorescence microscope with stage-top live-cell imaging chamber
(5) Compucyte iCys laser scanning cytometer
Training is provided for the use of the microscopes and software for processing and analysing microscope images. The software available includes Imaris, MetaMorph and Fiji.
Contact:
Dr Esther Koh (Head, Advanced Imaging Laboratory)
Email: lsikgle@nus.edu.sg
Phone: +65 6601 3152
Find out more on our website here.
Confocal Microscopy Unit
Yong Loo Lin School of Medicine, NUS
The Confocal Microscopy Unit in Yong Loo Lin School of Medicine is a multi-user facility that caters primarily to the imaging needs of the researchers or students from the faculty, whole of NUS as well as users outside of NUS.
We offer a range of microscopes for different experimental imaging needs (all microscopes are equipped with incubator for live-cell imaging work):
(1) Olympus FV1000 inverted confocal microscope (with TIRF)
(2) Olympus FV1000 inverted confocal microscope
(3) Olympus FV300 inverted confocal microscope
(4) Zeiss LSM710 inverted confocal microscope
(5) Zeiss Axio Observer inverted fluorescence microscope
(6) Zeiss LSM510 (upgrading to slide scanner with TissueGnostics)
We provide a one-stop solution for researchers imaging needs; such as
- sales of imaging chambers and reagents
- microscopy training
- software for image processing and analysis (Imaris, Metamorph, ImageJ, Fiji)
Contact:
Lee Shu Ying
Email: medleesy@nus.edu.sg
Phone: +65 6516 6306
Find out more on our website here.
Bioimaging & Biocomputing
Temasek Lifesciences Laboratory
The Bioimaging and Biocomputing facility provides TLL researchers with the instruments, resources, technical support and expertise necessary for a wide range of experiments. With our advanced light and electron microscopes, we can support a range of biological imaging. In addition, a High Performance Computing (HPC) cluster is available for bioinformatics analyses, as well as three Image Analysis workstations. As an integrated facility, we serve as a platform for biological data gathering and cellular informatics at TLL.
Light microscopes:
(1) Zeiss LSM510 Meta Inverted confocal microscope
(2) Zeiss LSM510 Meta Upright confocal microscope
(3) Zeiss LSM5 Exciter Upright confocal microscope
(4) Zeiss LSM510 Upright confocal microscope
(5) Leica SPE Upright confocal microscope
(6) 2 Spinning Disk confocal microscopes
(7) 3 Widefield Upright Fluorescence microscope
(8) 2 Widefield Inverted Fluorescence microscope
(9) 2 Leica Fluorescence Stereomicroscopes
Our resources are free-of-charge for TLL researchers and chargeable for any external users.
Contact:
Meredith Calvert (Head, Bioimaging and Biocomputing)
Email: meredith@tll.org.sg
Phone: +65 6872 8406
Find out more on our website here.
Microscopy Core
Mechanobiology Institute
Visualization of the dynamic biological processes rely on advanced imaging technologies. The Microscopy Core is the largest of the MBI core facilities, serving as the backbone for most of the experimental work at the Institute. The Core supports image related research, offering a wide range of microscopes and image analysis resources.
Some of the systems/techniques of particular interest to MBI which are present in our facility include:
(1) Fluorescence anisotropy
(2) Force measurements via optical tweezers
(3) Visualisation and quantification of micropillars deflection
(4) Single molecule studies
(5) Multiple TIRF setups
(6) FCS
(7) Week-long live cell imaging
(8) Laser ablation
(9) Super-resolution
All the above are in addition to our various standard widefield and confocal systems.
We are freely accessible to the MBI researchers and their collaborators. We are also open to the external community on a tiered chargeable basis. While training will be provided for the systems, guidance in initial system set up is available on demand.
Contact:
Felix Margadant (Facility Manager, MBI Microscopy Core)
Email: mbifmm@nus.edu.sg
Phone: +65 6601 1554
Find out more on our website here.
Advanced Bioimaging Core
Singapore Health Services Pte Ltd
SingHealth Advanced Bio-imaging aims to build up a Bio-imaging hub for both SingHealth and Singapore investigators by providing a wide variety of imaging platforms and services in both light and electron microscopy. We focus on super-resolution light microscopy, capable of conducting five types of imaging techniques:
(1) Structured Illumination Microscopy (SIM)
(2) Stimulated Emission Depletion microscopy (STED)
(3) Nikon-Stochastic Optical Reconstruction Microscopy (N-STORM)
(4) Direct-Stochastic Optical Reconstruction Microscopy (d-STORM)
(5) Photoactivated Localization Microscopy (PALM).
In addition we provide access services for many conventional imaging techniques in light microscopy:
(1) Bright field (BF).
(2) Differential Interference Contrast (DIC).
(3) Wide-Field fluorescence (WF).
(4) Total Internal Reflection Fluorescence (TIRF).
(5) Live-cell imaging (BF & WF),
(6) Laser scanning confocal microscopy (LSCM).
(7) High-Content Screening (HCS)
(8) Spinning Disk Confocal (SD)
For electron microscopy, the core is providing sample preparation and imaging services for transmission electron microscopy (TEM). The core facility currently owns a Joel JEM1200 TEM and EM preparation equipment from Leica.
For inquiries regarding imaging, training, and charging, please contact the core facility staff via email at bioimaging@singhealth.com.sg
Contacts:
Investigators are welcome to contact core facility management team for any collaboration possibilities:
Director: A/Prof Antonius Van Dongen
Email: antonius.vandongen@duke-nus.edu.sg
Phone: +65 6516 7075
Lab Manager: Dr. James Deqiang Zhao
Email: james.zhao.d.q@singhealth.com.sg
Phone: +65 6576 7186
Find out more on our website here.
Central Imaging Facility
Institute of Molecular and Cell Biology, A*STAR
IMCB CIF has light microscopes to visualize biological samples and acquire high-quality images. The various systems under CIF are used for different biological applications, such as bright-field imaging, phase contrast imaging, DIC (Normarski) imaging, wide-field and confocal fluorescence imaging. Training on using different systems and techniques is imparted to users.
CIF has a wide range of microscopes such as:
(1) Stereomicroscopes for sample screening
(2) Compound brightfield microscopes (Zeiss Axiovert 200M, Olympus BX61) for viewing and imaging of histological sections
(3) Compound wide-field microscopes (Leica DMI 6000, Zeiss Axioimager, Zeiss Axiovert 200M, Olympus BX61) fully-equipped for Live-Cell imaging
(4) Confocal laser microscopes (Olympus (FV1000) inverted & upright, Zeiss inverted (LSM700) and upright (LSM510 Meta) for 3D imaging and FRAP
(5) Multi-photon emission Confocal microscope for two-photon imaging
The facility is open to IMCB labs and IMCB CO-PI labs. External researchers can use on a payment basis.
Contact:
Dr Balakrishnan Kannan,
Email: imcbcif@imcb.a-star.edu.sg
Find out more on our webste here.
More microscopy facilities coming soon...
Advanced Biofilm Imaging Facility
Singapore Centre on Environmental Life Sciences Engineering (SCELSE), NTU
The Advanced Biofilm Imaging Facility is a core technology platform of the Singapore Centre on Environmental Life Sciences Engineering, NTU whose mission is to provide light microscopy equipment along with image processing and analysis software and the expertise to facilitate research in microbial cell biology and biofilm development.
We have a wide range of light microscopes such as:
(1) Zeiss ELYRA PS.1 + LSM780 inverted confocal microscope
(2) Zeiss LSM780 inverted confocal microscope
(3) Zeiss Axio Observer inverted fluorescence microscope
(4) Zeiss Axio Imager upright fluorescence microscope
(5) Zeiss Primostar
(6) Zeiss Discovery.V8 Stereoscope
In addition to standard widefield and confocal microscopy, our systems have capabilities to carry out several aspects of advanced imaging:
(1) Photoactivated Localization Microscopy (PALM)
(2) Direct-Stochastic Optical Reconstruction Microscopy (dSTORM)
(3) Superresolution Structured Illumination Microscopy (SR-SIM)
(4) Total Internal Reflection Fluorescence (TIRF) microscopy
(5) 3D imaging
(6) F-techniques
(7) High content imaging
(8) Live-cell imaging
(9) Image analysis & processing
Training is provided for the use of the microscopes and software for processing and analyzing microscope images. The software available includes Imaris, Zen and ImageJ/Fiji.
We are freely accessible to the SCELSE researchers, students and collaborators; and will soon be open to external users on a tiered charge-per-use basis.
Contact:
Melvin Sin (Facility Manager, Advanced Biofilm Imaging Facility)
Email: melvin.sin@ntu.edu.sg
Phone: +65 6592 7901
Find out more on out website here.